Higenamine 4′-O-β-d-glucoside in the lotus plumule induces glucose uptake of L6 cells through β2-adrenergic receptor

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Higenamine 4'-O-β-d-glucoside in the lotus plumule induces glucose uptake of L6 cells through β2-adrenergic receptor.

Hypoglycemic effect is an efficient means to modulate elevated blood glucose levels in patients with diabetes. We found that the extract of lotus plumule (the germ of Nelumbo nucifera Gaertn. seed) showed potent glucose uptake enhancement activity against L6 myotubes, which results in a hypoglycemic effect. This activity was further investigated, and an active constituent was identified as a si...

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Syringaresinol-4-O-β-d-glucoside (SSG), a furofuran-type lignan, was found to modulate lipid and glucose metabolism through an activity screen of lipid accumulation and glucose consumption, and was therefore considered as a promising candidate for the prevention and treatment of metabolic disorder, especially in lipid and glucose metabolic homeostasis. In this study, the effects of SSG on lipog...

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β-Adrenergic Inhibition of Contractility in L6 Skeletal Muscle Cells

The β-adrenoceptors (β-ARs) control many cellular processes. Here, we show that β-ARs inhibit calcium depletion-induced cell contractility and subsequent cell detachment of L6 skeletal muscle cells. The mechanism underlying the cell detachment inhibition was studied by using a quantitative cell detachment assay. We demonstrate that cell detachment induced by depletion of extracellular calcium i...

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The present study is an attempt to understand the role of vitamin D deficiency in pathogenesis of diabetes and put forth a possible explanation for its mechanism of action. Skeletal muscle cells (L6) were separately grown in media with very low vitamin D as well as optimized dose (10 -9 M) of vitamin D supplemented media.,. It was observed that L6 cells grown in presence of vitamin D (10 -9 M) ...

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Synthesis and Detailed Examination of Spectral Properties of (S) and (R)-Higenamine 4'-O-β-d-Glucoside and HPLC Analytical Conditions to Distinguish the Diastereomers.

Higenamine is a tetrahydroisoquinoline present in several plants that has β-adrenergic receptor agonist activity. Study of the biosynthesis of higenamine has shown the participation of norcoclaurine synthase, which controls the stereochemistry to construct the (S)-isomer. However, when isolated from nature, higenamine is found as the racemate, or even the (R)-isomer. We recently reported the is...

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ژورنال

عنوان ژورنال: Bioorganic & Medicinal Chemistry

سال: 2015

ISSN: 0968-0896

DOI: 10.1016/j.bmc.2015.04.054